White, Gaye F., Shi, Zhi, Shi, Liang, Dohnalkova, Alice C., Fredrickson, James K., Zachara, John M., Butt, Julea N.
ORCID: https://orcid.org/0000-0002-9624-5226, Richardson, David J. and Clarke, Thomas A.
(2012)
Development of a proteoliposome model to probe transmembrane electron-transfer reactions.
Biochemical Society Transactions, 40 (6).
pp. 1257-1260.
ISSN 0300-5127
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Abstract
The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.
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