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Zafrilla, Basilio, Martínez‑Espinosa, Rosa María, Bonete, Maria José, Butt, Julea N. 
ORCID: https://orcid.org/0000-0002-9624-5226, Richardson, David J. and Gates, Andrew J. ORCID: https://orcid.org/0000-0002-4594-5038
(2011)
A haloarchaeal ferredoxin electron donor that plays an essential role in nitrate assimilation.
Biochemical Society Transactions, 39 (6).
pp. 1844-1848.
ISSN 0300-5127
Abstract
In the absence of ammonium, many organisms, including the halophilic archaeon Haloferax volcanii DS2 (DM3757), may assimilate inorganic nitrogen from nitrate or nitrite, using a ferredoxin-dependent assimilatory NO3-/NO2- reductase pathway. The small acidic ferredoxin Hv-Fd plays an essential role in the electron transfer cascade required for assimilatory nitrate and nitrite reduction by the cytoplasmic NarB- and NirA-type reductases respectively. UV–visible absorbance and EPR spectroscopic characterization of purified Hv-Fd demonstrate that this protein binds a single [2Fe–2S] cluster, and potentiometric titration reveals that the cluster shares similar redox properties with those present in plant-type ferredoxins.
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