Tramonti, Daniela, Andrew, Elizabeth M, Rhodes, Kate, Newton, Darren J and Carding, Simon R (2006) Evidence for the opposing roles of different γδ T cell subsets in macrophage homeostasis. European Journal of Immunology, 36 (7). pp. 1729-1738. ISSN 0014-2980

Full text not available from this repository.

Abstract

To ensure invading pathogens are eliminated with minimal damage to host tissues it is essential that macrophage activation be tightly regulated. Previously we demonstrated that a subset of gammadelta T cells (Vgamma1(+)) contributes to resolving pathogen-induced immune responses by killing activated macrophages. However, the exaggerated macrophage response seen in infected Vgamma1(+) T cell-deficient mice suggests that gammadelta T cells play a broader role in macrophage homeostasis and other subsets might promote macrophage activation. Using a macrophage:gammadelta T cell co-culture system we have shown that gammadelta T cells increase the activity of macrophages activated in vivo by Listeria monocytogenes infection. In a dose-dependent manner, gammadelta T cells up-regulated production of cytokines (TNF-alpha, IL-6, IL-10) and chemokines (MIP-1alpha, MIP-1beta) by Listeria-elicited macrophages. The ability to increase macrophage cytokine production was prominent among Vgamma4(+) gammadelta T cells. Reciprocally, Vgamma4(+) gammadelta T cells were activated by Listeria-elicited macrophages, resulting in production of the anti-inflammatory cytokine, IL-10. gammadelta T cell adoptive transfer experiments showed that Vgamma4(+) T cells protected TCRdelta(-/-) mice against Listeria-induced liver injury and necrosis. These findings identify distinct and non-overlapping roles for gammadelta T cell subsets in regulating macrophage function during pathogen-induced immune responses.

Item Type:	Article
Uncontrolled Keywords:	animals,cells, cultured,chemokines,coculture techniques,dose-response relationship, immunologic,homeostasis,listeria monocytogenes,macrophages,mice,mice, inbred c57bl,mice, knockout,receptors, antigen, t-cell, gamma-delta,t-lymphocyte subsets
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Rhiannon Harvey
Date Deposited:	14 Jul 2011 11:40
Last Modified:	16 Jun 2026 13:07
URI:	https://uea-test-2026.eprints-hosting.org/id/eprint/33730
DOI:	10.1002/eji.200635959

Actions (login required)

View Item